The compact fluoroskan ascent cf is simplified and standardized to focus specifically on cellbased requirements. Windowsbased ascent software is designed to power all thermo. The thermo scientific fluoroskan ascent fl is a compact microplate fluorometer and luminometer. An optical system based in fiberless direct illumination gives the instrument a wide range of applications. Thermo scientific fluoroskan ascent the thermo scientific fluoroskan ascent is a microplate fluorometer for life science research applications, such as fluorometric protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, immuno and cell based assays. A wide range of accessories for specialized sample handling is available for the genesys 10s spectrophotometers.
The agilent 8453 is a diode array, ultraviolet, visible light and limited near infrared spectrophotometer. This high performing model is an advanced 96well microplate flurometer designed to read fluorescences from black or opaque fluoroplates. Ascent software features a clear and easytofollow approach with highly visual assay setup, userfriendly and highly sophisticated data handling, and. Perform a variety of research applications with the compact, robust thermo scientific. Up to three reagent dispensers can be fitted onboard, making the addition simple and highly accurate. The fluoroskan fl microplate fluorometer and luminometer is capable of reading a 96well plate in just 18 seconds, essential for kinetic applications. Ascent software features a clear and easytofollow approach with highly visual assay setup, userfriendly and highly sophisticated data handling, and effective integration to automation and lims systems. Perform a number of fluorometric and luminometric research applications with the thermo scientific fluoroskan ascent fl microplate fluorometer and luminometer, which offers versatile plate. Thermo fluoroskan ascent fl ideal for life science research applications. Fluorometric gfp measurement and luminometric luciferase measurement from the same well occurs quickly due to the versatility of the assay programming. The data was analyzed with ascent software that automatically calculated the genoxcytox ratio.
Government prime contract or subcontract, software is delivered and licensed as commercial computer software as defined in dfar 252. Control software bmg labtech 28 0470f0026a 20100315 this manual was designed to guide pherastar users through the software features. Thermo electron corporation fluoroskan ascent model 374. This high performing model is an advanced 96well microplate flurometer designed to read fluorescences from black or. Thermo scientific fluoroskan ascent fl from thermo fisher. Reversal of rivaroxaban and dabigatran by prothrombin complex. Fluoresence microplate reader with 320 to 700nm excitation and 360 to 670nm emission wavelength capabilities.
The fluoroskan ascent is a fluorometer for 96well and 384well microplates. Fluoroskan fl microplate fluorometer and luminometer. In fluorescence measurement, this prevents crosstalk and ensures accurate readings. Mar 10, 2019 thermo scientific fluoroskan ascent microplate fluorometer. The calibrated automated thrombogram assays the generation of thrombin in clotting plasma using a microtiter platereading fluorometer fluoroskan ascent, thermolab systems, helsinki, finland and thrombinoscope software thrombinoscope bv, maastricht, the netherlands. The information contained within this technical bulletin applies only to corning fluoroblok insert systems and corning biocoat multiwell insert cellbased assays i. The multiskan ex comes with flexible internal software. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic. Stateoftheart optical system for unrivalled sensitivity highlyvisual ascent software simplifies fluorometric and luminometric applications ascent fl onboard precision dispensers various models for rapid kinetic measurements on fl model only flexible plate formats, from 1.
Thermo scientific ascent software from thermo fisher. Fluoroskan ascent fl and ascent software support simultaneous dispensing and reading, enabling aequorin signals to be monitored from the start of the reaction. Ascent business systems field service management software. Thermo scientific fluoroskan fl, fluoroskan and luminoskan.
Although these instructions were carefully written and checked, we cannot accept responsibility for problems encountered when using this manual. Use, duplication or disclosure of software is subject to agilent technologies standard commercial license terms, and nondod. Ascent software has a special remote control interface for robotic use which enables easy integration with robotics and lims systems. Thermo ascent software flexible, easytouse software to control all members of the ascent microplate reader family. Thermo scientific luminoskan ascent thermo scientific. This reader is fast and accurate, reading the whole plate in only 5 seconds. Thermo scientific thermo scientific fluoroskan fl, fluoroskan and luminoskan user manual i p preface about this guide thermo scientific fluoroskan fl, fluoroskan and luminoskan are microplate readers and they are controlled by thermo scientific skanit software for microplate readers. Thermo labsystems fluoroskan ascent fl fluoresence microplate reader description. Multiskan ascent 96384 plate reader with ascent software, and user manuals multiskan ascent offers superior photometric measurement performance for 96 and 384well plates. The program searches for wells to dispensemeasure in the order defined by this input field. The optical system of the multiskan ex meets the requirements of current eia technology, offering a linear measurement range up to 3.
Used thermo fluoroskan ascent 374 fluorometer for sale. This guide also covers the upgrade procedure from ascent software to skanit software. Labsystems fluoroskan ii fluorescent microplate reader gmi. Shop online for a wide selection of thermo scientific fluoroskan ascent microplate fluorometer perform a variety of research applications with the. Please consult the instrument user manual to obtain key reference points and units. Fisherbrand accuskan fc filterbased microplate photometer. Ascent software controls all the reader functions and provides easy assay. Perkinelmer hts 7000, tecan spectrafluor plus, and thermo labsystems fluoroskan ascent.
From challenge analysis to product design, to devopspowered cicd automation, to cloudfirst experiences, to machine learning, to ai, and automated testing, ascent enables you to create a digital advantage of software that can be. Application areas of fluoroskan ascent fl atp detection. Labsystems fluoroskan ascent microplate luminometer combo. Thermo labsystems fluoroskan ascent fl fluoresence microplate.
The fluoroskan ascent fl is equipped with both fluorometric and luminometric measurement technologies, providing a full coverage for fluorometric, glow and flash luminometric applications even in the same run. With 30 years of experience solving operational business challenges, our offering of solutions and best practice business knowledge will provide you with a competitive edge. Thermo scientific fluoroskan ascent microplate fluorometer. Reversal of rivaroxaban and dabigatran by prothrombin. For further information about the features of the iqoqpq, see page 52. Fluoroskan ascent microplate fluorometer from thermo. Operation the luminoskan ascent is fully computercontrolled. Fluorometric gfp measurement and luminometric luciferase measurement from the same well occurs quickly due to. The plate carrier allows convenient access for robotic arm and ascent software is easy to integrate with his li ms systems. Unit is used, does not include software or further accessories.
Ascent software windowsbased ascent software is designed to power all thermo. Thermo scientific fluoroskan fl, fluoroskan and luminoskan technical manual cat. Parts for fluoroskan ascent from thermo scientific brand microplate readers offered by unity lab services, part of thermo fisher scientific. Whichever ascent microplate instrument you use, the highly visual ascent software always looks the same, providing familiar and flexible control. Clear and easytofollow approach ascent software is divided into two. Thermo scientific skanit software for microplate readers. The manual for fluoroskan ascent can be found on thermophile website. Choose cell holders for vials or long path cells, add a sipper kit to eliminate handling of cuvettes, or thermostat your sample with circulating water or an aircooled peltier accessory.
Thermo scientific fluoroskan ascent microplate fluorometer is a compact and robust instrument with excellent optical performance for a variety of research applications. The direct illumination optics of the fluoroskan ascent produces a highly focused light beam. The combination of the accuskan fc microplate photometer and the accuwash microplate washer offers a convenient modular solution for routine and research elisa applications. Thus, the reader is ideal for applications such as reporter gene and cell adhesion assays, as well as cell proliferation and cytotoxicity assays. No description 5185410cd ascent software for fluoroskan ascent fl 5185430cd ascent software for multiskan ex 5185450cd ascent software for luminoskan ascent ascent software. This instrument can record data from 190 to 1100nm. The information contained within this technical bulletin applies only to. Five ml of the s9 mix or purified water and 40ml of the bacterial cultures were added to each well. In addition to its accurate measurements, the fluoroskan ascent has userfriendly software for easy analysis of every reading. Instruments come with 1 filter pairs of choice, manual, line cord and three year warranty. The labsystems fluoroskan ii fluorescent is a microplate reader crafted to answer the most discriminate needs of any research and clinical laboratory. Tecan spectrafluor plus, and thermo labsystems fluoroskan. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic measurements from the very start of the reaction. The assay was carried out as described by hemker et al25 and the thrombinoscope manual.
Labsystems fluoroskan ii fluorescent microplate reader. Fluoroskan fl is a combination instrument and in cludes both fluorometry and luminometry, whereas. A proven elisa solutionaccuskan fc and the fisher scientific accuwash washer. Fluoroskan ascent, 100240 v 14230352 fluoroskan ascent, 100240 v, with one dispenser 14230354 fluoroskan ascent, 100240 v, with two dispensers 95043042 fluoroskan ascent, 100240 v, with three dispensers 95043044 fluoroskan ascent fl, 100240 v 14230346 fluoroskan ascent fl, 100240 v, with one dispenser 14230348.
Perform a number of fluorometric and luminometric research applications with the thermo scientific fluoroskan ascent fl microplate fluorometer and luminometer, which offers versatile plate formats, fast plate reading speeds, topbottom plate reading and up to three dispensers. Labsystems fluoroskan ascent, bmg fluostar, wallac victor 2, tecan genios1 and others. For further details on how to use the software, refer to the ascent software users guide. Skanit software upgrade package is also compatible with the discontinued fluoroskan ascent, fluoroskan ascent fl, and luminoskan ascent microplate readers. Ascent software, fluoroskan, fluoroskan ascent, immulon, luminoskan.
This instrument reads the plate rapidly and can be connected to robotic systems for increased throughput in photometric reading. Thermo electron ascent software controls the reader functions and provides complete data handling and report formatting. Up to three reagent dispensers can be fitted onboard, making the reagent addition simple and highly accurate. Related documentation in addition to this guide, thermo fisher scientific provides the following documents for fluoroskan fl, fluoroskan, luminoskan and skanit software. Fluoroskan ascent, thermolab systems, helsinki, finland and thrombinoscope software thrombinoscope bv, maastricht, the netherlands. Fluoroskan ascent fluorescence cf cell reading fluorescence.